ppcr-script cam+ cloning vector (Agilent technologies)
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Agilent technologies
ppcr-script cam+ cloning vector
Ppcr Script Cam+ Cloning Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ppcr-script+vector/pm37834049-458-2-6
Average 90 stars, based on 1 article reviews
Ppcr Script Cam+ Cloning Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ppcr-script+vector/pm37834049-458-2-6
Average 90 stars, based on 1 article reviews
ppcr-script cam+ cloning vector - by Bioz Stars,
2026-11
90/100 stars
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Synthesized:Article Title: Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid Article Snippet: The amplified promoter fragment was subsequently cloned into the Article Title: Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy. Article Snippet: A stop codon was removed from Cxcr4b gene previously cloned in a Article Title: Pentaplexed Quantitative Real-Time PCR Assay for the Simultaneous Detection and Quantification of Botulinum Neurotoxin-Producing Clostridia in Food and Clinical Samples Article Snippet: The IAC consists of a chemically synthesized DNA sequence (Table ) containing the target sequence for the primers KoMa_F and KoMa_R and the probes KoMa_TM or KoMa_TS (Table ) cloned into the Article Title: The Fertilization-Induced DNA Replication Factor MCM6 of Maize Shuttles between Cytoplasm and Nucleus, and Is Essential for Plant Growth and Development Article Snippet: PCR products were blunted, purified, and cloned into the Article Title: Genetic and Genomic Analysis of the AT-Rich Centromere DNA Element II of Saccharomyces cerevisiae Article Snippet: The product was ligated into Article Title: Tri-split tRNA is a transfer RNA made from 3 transcripts that provides insight into the evolution of fragmented tRNAs in archaea Article Snippet: RT-PCR products were further purified by using an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare) and subcloned into the Srf I-digested Article Title: Self-excision of the antibiotic resistance gene nptII using a heat inducible Cre-loxP system from transgenic potato. Article Snippet: Resistance to antibiotics mediated by selectable marker genes remains a powerful selection tool for transgenic event production.. However, regulatory agencies and consumer concerns favor these to be eliminated from food crops.. Several excision systems exist but none have been optimized or shown to be functional for clonally propagated crops. Article Title: Activation of LXR increases acyl-CoA synthetase activity through direct regulation of ACSL3 in human placental trophoblast cells Article Snippet: The full-length human ACSL3 reporter construct [ACSL3 (−2189/+213)LUC] was generated as described previously ( 28 ) using pPCR-Script vector (Stratagene, La Jolla, CA) prior to insertion into the BglII site in the pGL3-Basic luciferase reporter vector (Promega, Madison, WI). Sequencing:Article Title: Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid Article Snippet: The amplified promoter fragment was subsequently cloned into the Article Title: Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy. Article Snippet: A stop codon was removed from Cxcr4b gene previously cloned in a Article Title: Pentaplexed Quantitative Real-Time PCR Assay for the Simultaneous Detection and Quantification of Botulinum Neurotoxin-Producing Clostridia in Food and Clinical Samples Article Snippet: The IAC consists of a chemically synthesized DNA sequence (Table ) containing the target sequence for the primers KoMa_F and KoMa_R and the probes KoMa_TM or KoMa_TS (Table ) cloned into the Article Title: The Fertilization-Induced DNA Replication Factor MCM6 of Maize Shuttles between Cytoplasm and Nucleus, and Is Essential for Plant Growth and Development Article Snippet: PCR products were blunted, purified, and cloned into the Article Title: Genetic and Genomic Analysis of the AT-Rich Centromere DNA Element II of Saccharomyces cerevisiae Article Snippet: The product was ligated into Article Title: Tri-split tRNA is a transfer RNA made from 3 transcripts that provides insight into the evolution of fragmented tRNAs in archaea Article Snippet: RT-PCR products were further purified by using an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare) and subcloned into the Srf I-digested Article Title: Self-excision of the antibiotic resistance gene nptII using a heat inducible Cre-loxP system from transgenic potato. Article Snippet: Resistance to antibiotics mediated by selectable marker genes remains a powerful selection tool for transgenic event production.. However, regulatory agencies and consumer concerns favor these to be eliminated from food crops.. Several excision systems exist but none have been optimized or shown to be functional for clonally propagated crops. Article Title: Activation of LXR increases acyl-CoA synthetase activity through direct regulation of ACSL3 in human placental trophoblast cells Article Snippet: The full-length human ACSL3 reporter construct [ACSL3 (−2189/+213)LUC] was generated as described previously ( 28 ) using pPCR-Script vector (Stratagene, La Jolla, CA) prior to insertion into the BglII site in the pGL3-Basic luciferase reporter vector (Promega, Madison, WI). Clone Assay:Article Title: Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid Article Snippet: The amplified promoter fragment was subsequently cloned into the Article Title: Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy. Article Snippet: A stop codon was removed from Cxcr4b gene previously cloned in a Article Title: Pentaplexed Quantitative Real-Time PCR Assay for the Simultaneous Detection and Quantification of Botulinum Neurotoxin-Producing Clostridia in Food and Clinical Samples Article Snippet: The IAC consists of a chemically synthesized DNA sequence (Table ) containing the target sequence for the primers KoMa_F and KoMa_R and the probes KoMa_TM or KoMa_TS (Table ) cloned into the Article Title: The Fertilization-Induced DNA Replication Factor MCM6 of Maize Shuttles between Cytoplasm and Nucleus, and Is Essential for Plant Growth and Development Article Snippet: PCR products were blunted, purified, and cloned into the Article Title: Genetic and Genomic Analysis of the AT-Rich Centromere DNA Element II of Saccharomyces cerevisiae Article Snippet: The product was ligated into Article Title: Tri-split tRNA is a transfer RNA made from 3 transcripts that provides insight into the evolution of fragmented tRNAs in archaea Article Snippet: RT-PCR products were further purified by using an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare) and subcloned into the Srf I-digested Article Title: Self-excision of the antibiotic resistance gene nptII using a heat inducible Cre-loxP system from transgenic potato. Article Snippet: Resistance to antibiotics mediated by selectable marker genes remains a powerful selection tool for transgenic event production.. However, regulatory agencies and consumer concerns favor these to be eliminated from food crops.. Several excision systems exist but none have been optimized or shown to be functional for clonally propagated crops. Article Title: Activation of LXR increases acyl-CoA synthetase activity through direct regulation of ACSL3 in human placental trophoblast cells Article Snippet: The full-length human ACSL3 reporter construct [ACSL3 (−2189/+213)LUC] was generated as described previously ( 28 ) using pPCR-Script vector (Stratagene, La Jolla, CA) prior to insertion into the BglII site in the pGL3-Basic luciferase reporter vector (Promega, Madison, WI). Plasmid Preparation:Article Title: Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid Article Snippet: The amplified promoter fragment was subsequently cloned into the Article Title: Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy. Article Snippet: A stop codon was removed from Cxcr4b gene previously cloned in a Article Title: Pentaplexed Quantitative Real-Time PCR Assay for the Simultaneous Detection and Quantification of Botulinum Neurotoxin-Producing Clostridia in Food and Clinical Samples Article Snippet: The IAC consists of a chemically synthesized DNA sequence (Table ) containing the target sequence for the primers KoMa_F and KoMa_R and the probes KoMa_TM or KoMa_TS (Table ) cloned into the Article Title: The Fertilization-Induced DNA Replication Factor MCM6 of Maize Shuttles between Cytoplasm and Nucleus, and Is Essential for Plant Growth and Development Article Snippet: PCR products were blunted, purified, and cloned into the Article Title: Genetic and Genomic Analysis of the AT-Rich Centromere DNA Element II of Saccharomyces cerevisiae Article Snippet: The product was ligated into Article Title: Tri-split tRNA is a transfer RNA made from 3 transcripts that provides insight into the evolution of fragmented tRNAs in archaea Article Snippet: RT-PCR products were further purified by using an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare) and subcloned into the Srf I-digested Article Title: Self-excision of the antibiotic resistance gene nptII using a heat inducible Cre-loxP system from transgenic potato. Article Snippet: Resistance to antibiotics mediated by selectable marker genes remains a powerful selection tool for transgenic event production.. However, regulatory agencies and consumer concerns favor these to be eliminated from food crops.. Several excision systems exist but none have been optimized or shown to be functional for clonally propagated crops. Article Title: Activation of LXR increases acyl-CoA synthetase activity through direct regulation of ACSL3 in human placental trophoblast cells Article Snippet: The full-length human ACSL3 reporter construct [ACSL3 (−2189/+213)LUC] was generated as described previously ( 28 ) using pPCR-Script vector (Stratagene, La Jolla, CA) prior to insertion into the BglII site in the pGL3-Basic luciferase reporter vector (Promega, Madison, WI). Construct:Article Title: Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid Article Snippet: The amplified promoter fragment was subsequently cloned into the Article Title: Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy. Article Snippet: A stop codon was removed from Cxcr4b gene previously cloned in a Article Title: Pentaplexed Quantitative Real-Time PCR Assay for the Simultaneous Detection and Quantification of Botulinum Neurotoxin-Producing Clostridia in Food and Clinical Samples Article Snippet: The IAC consists of a chemically synthesized DNA sequence (Table ) containing the target sequence for the primers KoMa_F and KoMa_R and the probes KoMa_TM or KoMa_TS (Table ) cloned into the Article Title: The Fertilization-Induced DNA Replication Factor MCM6 of Maize Shuttles between Cytoplasm and Nucleus, and Is Essential for Plant Growth and Development Article Snippet: PCR products were blunted, purified, and cloned into the Article Title: Genetic and Genomic Analysis of the AT-Rich Centromere DNA Element II of Saccharomyces cerevisiae Article Snippet: The product was ligated into Article Title: Tri-split tRNA is a transfer RNA made from 3 transcripts that provides insight into the evolution of fragmented tRNAs in archaea Article Snippet: RT-PCR products were further purified by using an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare) and subcloned into the Srf I-digested Article Title: Self-excision of the antibiotic resistance gene nptII using a heat inducible Cre-loxP system from transgenic potato. Article Snippet: Resistance to antibiotics mediated by selectable marker genes remains a powerful selection tool for transgenic event production.. However, regulatory agencies and consumer concerns favor these to be eliminated from food crops.. Several excision systems exist but none have been optimized or shown to be functional for clonally propagated crops. Article Title: Activation of LXR increases acyl-CoA synthetase activity through direct regulation of ACSL3 in human placental trophoblast cells Article Snippet: The full-length human ACSL3 reporter construct [ACSL3 (−2189/+213)LUC] was generated as described previously ( 28 ) using pPCR-Script vector (Stratagene, La Jolla, CA) prior to insertion into the BglII site in the pGL3-Basic luciferase reporter vector (Promega, Madison, WI). Generated:Article Title: Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid Article Snippet: The amplified promoter fragment was subsequently cloned into the Article Title: Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy. Article Snippet: A stop codon was removed from Cxcr4b gene previously cloned in a Article Title: Pentaplexed Quantitative Real-Time PCR Assay for the Simultaneous Detection and Quantification of Botulinum Neurotoxin-Producing Clostridia in Food and Clinical Samples Article Snippet: The IAC consists of a chemically synthesized DNA sequence (Table ) containing the target sequence for the primers KoMa_F and KoMa_R and the probes KoMa_TM or KoMa_TS (Table ) cloned into the Article Title: The Fertilization-Induced DNA Replication Factor MCM6 of Maize Shuttles between Cytoplasm and Nucleus, and Is Essential for Plant Growth and Development Article Snippet: PCR products were blunted, purified, and cloned into the Article Title: Genetic and Genomic Analysis of the AT-Rich Centromere DNA Element II of Saccharomyces cerevisiae Article Snippet: The product was ligated into Article Title: Tri-split tRNA is a transfer RNA made from 3 transcripts that provides insight into the evolution of fragmented tRNAs in archaea Article Snippet: RT-PCR products were further purified by using an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare) and subcloned into the Srf I-digested Article Title: Self-excision of the antibiotic resistance gene nptII using a heat inducible Cre-loxP system from transgenic potato. Article Snippet: Resistance to antibiotics mediated by selectable marker genes remains a powerful selection tool for transgenic event production.. However, regulatory agencies and consumer concerns favor these to be eliminated from food crops.. Several excision systems exist but none have been optimized or shown to be functional for clonally propagated crops. Article Title: Activation of LXR increases acyl-CoA synthetase activity through direct regulation of ACSL3 in human placental trophoblast cells Article Snippet: The full-length human ACSL3 reporter construct [ACSL3 (−2189/+213)LUC] was generated as described previously ( 28 ) using pPCR-Script vector (Stratagene, La Jolla, CA) prior to insertion into the BglII site in the pGL3-Basic luciferase reporter vector (Promega, Madison, WI). Luciferase:Article Title: Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid Article Snippet: The amplified promoter fragment was subsequently cloned into the Article Title: Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy. Article Snippet: A stop codon was removed from Cxcr4b gene previously cloned in a Article Title: Pentaplexed Quantitative Real-Time PCR Assay for the Simultaneous Detection and Quantification of Botulinum Neurotoxin-Producing Clostridia in Food and Clinical Samples Article Snippet: The IAC consists of a chemically synthesized DNA sequence (Table ) containing the target sequence for the primers KoMa_F and KoMa_R and the probes KoMa_TM or KoMa_TS (Table ) cloned into the Article Title: The Fertilization-Induced DNA Replication Factor MCM6 of Maize Shuttles between Cytoplasm and Nucleus, and Is Essential for Plant Growth and Development Article Snippet: PCR products were blunted, purified, and cloned into the Article Title: Genetic and Genomic Analysis of the AT-Rich Centromere DNA Element II of Saccharomyces cerevisiae Article Snippet: The product was ligated into Article Title: Tri-split tRNA is a transfer RNA made from 3 transcripts that provides insight into the evolution of fragmented tRNAs in archaea Article Snippet: RT-PCR products were further purified by using an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare) and subcloned into the Srf I-digested Article Title: Self-excision of the antibiotic resistance gene nptII using a heat inducible Cre-loxP system from transgenic potato. Article Snippet: Resistance to antibiotics mediated by selectable marker genes remains a powerful selection tool for transgenic event production.. However, regulatory agencies and consumer concerns favor these to be eliminated from food crops.. Several excision systems exist but none have been optimized or shown to be functional for clonally propagated crops. Article Title: Activation of LXR increases acyl-CoA synthetase activity through direct regulation of ACSL3 in human placental trophoblast cells Article Snippet: The full-length human ACSL3 reporter construct [ACSL3 (−2189/+213)LUC] was generated as described previously ( 28 ) using pPCR-Script vector (Stratagene, La Jolla, CA) prior to insertion into the BglII site in the pGL3-Basic luciferase reporter vector (Promega, Madison, WI). Reverse Transcription Polymerase Chain Reaction:Article Title: Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid Article Snippet: The amplified promoter fragment was subsequently cloned into the Article Title: Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy. Article Snippet: A stop codon was removed from Cxcr4b gene previously cloned in a Article Title: Pentaplexed Quantitative Real-Time PCR Assay for the Simultaneous Detection and Quantification of Botulinum Neurotoxin-Producing Clostridia in Food and Clinical Samples Article Snippet: The IAC consists of a chemically synthesized DNA sequence (Table ) containing the target sequence for the primers KoMa_F and KoMa_R and the probes KoMa_TM or KoMa_TS (Table ) cloned into the Article Title: The Fertilization-Induced DNA Replication Factor MCM6 of Maize Shuttles between Cytoplasm and Nucleus, and Is Essential for Plant Growth and Development Article Snippet: PCR products were blunted, purified, and cloned into the Article Title: Genetic and Genomic Analysis of the AT-Rich Centromere DNA Element II of Saccharomyces cerevisiae Article Snippet: The product was ligated into Article Title: Tri-split tRNA is a transfer RNA made from 3 transcripts that provides insight into the evolution of fragmented tRNAs in archaea Article Snippet: RT-PCR products were further purified by using an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare) and subcloned into the Srf I-digested Article Title: Self-excision of the antibiotic resistance gene nptII using a heat inducible Cre-loxP system from transgenic potato. Article Snippet: Resistance to antibiotics mediated by selectable marker genes remains a powerful selection tool for transgenic event production.. However, regulatory agencies and consumer concerns favor these to be eliminated from food crops.. Several excision systems exist but none have been optimized or shown to be functional for clonally propagated crops. Article Title: Activation of LXR increases acyl-CoA synthetase activity through direct regulation of ACSL3 in human placental trophoblast cells Article Snippet: The full-length human ACSL3 reporter construct [ACSL3 (−2189/+213)LUC] was generated as described previously ( 28 ) using pPCR-Script vector (Stratagene, La Jolla, CA) prior to insertion into the BglII site in the pGL3-Basic luciferase reporter vector (Promega, Madison, WI). Purification:Article Title: Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid Article Snippet: The amplified promoter fragment was subsequently cloned into the Article Title: Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy. Article Snippet: A stop codon was removed from Cxcr4b gene previously cloned in a Article Title: Pentaplexed Quantitative Real-Time PCR Assay for the Simultaneous Detection and Quantification of Botulinum Neurotoxin-Producing Clostridia in Food and Clinical Samples Article Snippet: The IAC consists of a chemically synthesized DNA sequence (Table ) containing the target sequence for the primers KoMa_F and KoMa_R and the probes KoMa_TM or KoMa_TS (Table ) cloned into the Article Title: The Fertilization-Induced DNA Replication Factor MCM6 of Maize Shuttles between Cytoplasm and Nucleus, and Is Essential for Plant Growth and Development Article Snippet: PCR products were blunted, purified, and cloned into the Article Title: Genetic and Genomic Analysis of the AT-Rich Centromere DNA Element II of Saccharomyces cerevisiae Article Snippet: The product was ligated into Article Title: Tri-split tRNA is a transfer RNA made from 3 transcripts that provides insight into the evolution of fragmented tRNAs in archaea Article Snippet: RT-PCR products were further purified by using an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare) and subcloned into the Srf I-digested Article Title: Self-excision of the antibiotic resistance gene nptII using a heat inducible Cre-loxP system from transgenic potato. Article Snippet: Resistance to antibiotics mediated by selectable marker genes remains a powerful selection tool for transgenic event production.. However, regulatory agencies and consumer concerns favor these to be eliminated from food crops.. Several excision systems exist but none have been optimized or shown to be functional for clonally propagated crops. Article Title: Activation of LXR increases acyl-CoA synthetase activity through direct regulation of ACSL3 in human placental trophoblast cells Article Snippet: The full-length human ACSL3 reporter construct [ACSL3 (−2189/+213)LUC] was generated as described previously ( 28 ) using pPCR-Script vector (Stratagene, La Jolla, CA) prior to insertion into the BglII site in the pGL3-Basic luciferase reporter vector (Promega, Madison, WI). Polymerase Chain Reaction:Article Title: Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid Article Snippet: The amplified promoter fragment was subsequently cloned into the Article Title: Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy. Article Snippet: A stop codon was removed from Cxcr4b gene previously cloned in a Article Title: Pentaplexed Quantitative Real-Time PCR Assay for the Simultaneous Detection and Quantification of Botulinum Neurotoxin-Producing Clostridia in Food and Clinical Samples Article Snippet: The IAC consists of a chemically synthesized DNA sequence (Table ) containing the target sequence for the primers KoMa_F and KoMa_R and the probes KoMa_TM or KoMa_TS (Table ) cloned into the Article Title: The Fertilization-Induced DNA Replication Factor MCM6 of Maize Shuttles between Cytoplasm and Nucleus, and Is Essential for Plant Growth and Development Article Snippet: PCR products were blunted, purified, and cloned into the Article Title: Genetic and Genomic Analysis of the AT-Rich Centromere DNA Element II of Saccharomyces cerevisiae Article Snippet: The product was ligated into Article Title: Tri-split tRNA is a transfer RNA made from 3 transcripts that provides insight into the evolution of fragmented tRNAs in archaea Article Snippet: RT-PCR products were further purified by using an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare) and subcloned into the Srf I-digested Article Title: Self-excision of the antibiotic resistance gene nptII using a heat inducible Cre-loxP system from transgenic potato. Article Snippet: Resistance to antibiotics mediated by selectable marker genes remains a powerful selection tool for transgenic event production.. However, regulatory agencies and consumer concerns favor these to be eliminated from food crops.. Several excision systems exist but none have been optimized or shown to be functional for clonally propagated crops. Article Title: Activation of LXR increases acyl-CoA synthetase activity through direct regulation of ACSL3 in human placental trophoblast cells Article Snippet: The full-length human ACSL3 reporter construct [ACSL3 (−2189/+213)LUC] was generated as described previously ( 28 ) using pPCR-Script vector (Stratagene, La Jolla, CA) prior to insertion into the BglII site in the pGL3-Basic luciferase reporter vector (Promega, Madison, WI). Transformation Assay:Article Title: Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid Article Snippet: The amplified promoter fragment was subsequently cloned into the Article Title: Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy. Article Snippet: A stop codon was removed from Cxcr4b gene previously cloned in a Article Title: Pentaplexed Quantitative Real-Time PCR Assay for the Simultaneous Detection and Quantification of Botulinum Neurotoxin-Producing Clostridia in Food and Clinical Samples Article Snippet: The IAC consists of a chemically synthesized DNA sequence (Table ) containing the target sequence for the primers KoMa_F and KoMa_R and the probes KoMa_TM or KoMa_TS (Table ) cloned into the Article Title: The Fertilization-Induced DNA Replication Factor MCM6 of Maize Shuttles between Cytoplasm and Nucleus, and Is Essential for Plant Growth and Development Article Snippet: PCR products were blunted, purified, and cloned into the Article Title: Genetic and Genomic Analysis of the AT-Rich Centromere DNA Element II of Saccharomyces cerevisiae Article Snippet: The product was ligated into Article Title: Tri-split tRNA is a transfer RNA made from 3 transcripts that provides insight into the evolution of fragmented tRNAs in archaea Article Snippet: RT-PCR products were further purified by using an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare) and subcloned into the Srf I-digested Article Title: Self-excision of the antibiotic resistance gene nptII using a heat inducible Cre-loxP system from transgenic potato. Article Snippet: Resistance to antibiotics mediated by selectable marker genes remains a powerful selection tool for transgenic event production.. However, regulatory agencies and consumer concerns favor these to be eliminated from food crops.. Several excision systems exist but none have been optimized or shown to be functional for clonally propagated crops. Article Title: Activation of LXR increases acyl-CoA synthetase activity through direct regulation of ACSL3 in human placental trophoblast cells Article Snippet: The full-length human ACSL3 reporter construct [ACSL3 (−2189/+213)LUC] was generated as described previously ( 28 ) using pPCR-Script vector (Stratagene, La Jolla, CA) prior to insertion into the BglII site in the pGL3-Basic luciferase reporter vector (Promega, Madison, WI). Amplification:Article Title: Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid Article Snippet: The amplified promoter fragment was subsequently cloned into the Article Title: Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy. Article Snippet: A stop codon was removed from Cxcr4b gene previously cloned in a Article Title: Pentaplexed Quantitative Real-Time PCR Assay for the Simultaneous Detection and Quantification of Botulinum Neurotoxin-Producing Clostridia in Food and Clinical Samples Article Snippet: The IAC consists of a chemically synthesized DNA sequence (Table ) containing the target sequence for the primers KoMa_F and KoMa_R and the probes KoMa_TM or KoMa_TS (Table ) cloned into the Article Title: The Fertilization-Induced DNA Replication Factor MCM6 of Maize Shuttles between Cytoplasm and Nucleus, and Is Essential for Plant Growth and Development Article Snippet: PCR products were blunted, purified, and cloned into the Article Title: Genetic and Genomic Analysis of the AT-Rich Centromere DNA Element II of Saccharomyces cerevisiae Article Snippet: The product was ligated into Article Title: Tri-split tRNA is a transfer RNA made from 3 transcripts that provides insight into the evolution of fragmented tRNAs in archaea Article Snippet: RT-PCR products were further purified by using an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare) and subcloned into the Srf I-digested Article Title: Self-excision of the antibiotic resistance gene nptII using a heat inducible Cre-loxP system from transgenic potato. Article Snippet: Resistance to antibiotics mediated by selectable marker genes remains a powerful selection tool for transgenic event production.. However, regulatory agencies and consumer concerns favor these to be eliminated from food crops.. Several excision systems exist but none have been optimized or shown to be functional for clonally propagated crops. Article Title: Activation of LXR increases acyl-CoA synthetase activity through direct regulation of ACSL3 in human placental trophoblast cells Article Snippet: The full-length human ACSL3 reporter construct [ACSL3 (−2189/+213)LUC] was generated as described previously ( 28 ) using pPCR-Script vector (Stratagene, La Jolla, CA) prior to insertion into the BglII site in the pGL3-Basic luciferase reporter vector (Promega, Madison, WI). |